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rabbit anti human p21 polyclonal antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit anti human p21 polyclonal antibody
    Senescence induction in BC cells following OxxySlab lysate treatment. Cell lines were treated with OxxySlab lysate (75–150 μg/mL) for 48 h. Senescent cells were identified by β-galactosidase staining (β-gal). Representative phase-contrast images of T24 ( A ), 5637 ( E ) and SV-HUC-1 ( I ) β-gal-positive cells (blue staining) are shown. Quantification of senescence was performed by measuring the Mean Gray Value of blue staining intensity using ImageJ software, with data expressed as Inverted Mean Gray Value (255 − MGV), which directly reflects β-gal activity. Data are expressed as mean ± SEM of two independent experiments in triplicate. Western blot analysis of the senescence markers <t>p21,</t> p53 and p16 was performed in untreated (CNTR) and OxxySlab -treated T24 ( B – D ), 5637 ( F – H ), and SV-HUC1 ( J – L ) cells. Protein levels were quantified by densitometry, normalized to GAPDH, and expressed as fold change relative to CNTR of three independent experiments (mean ± SEM). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01).
    Rabbit Anti Human P21 Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+p21+polyclonal+antibody/p21/pmc12649232-92-59-64
    Average 96 stars, based on 5650 article reviews
    rabbit anti human p21 polyclonal antibody - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Exploring the Anticancer Potential of the Multistrain Probiotic Formulation OxxySlab in Bladder Cancer Cell Lines"

    Article Title: Exploring the Anticancer Potential of the Multistrain Probiotic Formulation OxxySlab in Bladder Cancer Cell Lines

    Journal: Antioxidants

    doi: 10.3390/antiox14111282

    Senescence induction in BC cells following OxxySlab lysate treatment. Cell lines were treated with OxxySlab lysate (75–150 μg/mL) for 48 h. Senescent cells were identified by β-galactosidase staining (β-gal). Representative phase-contrast images of T24 ( A ), 5637 ( E ) and SV-HUC-1 ( I ) β-gal-positive cells (blue staining) are shown. Quantification of senescence was performed by measuring the Mean Gray Value of blue staining intensity using ImageJ software, with data expressed as Inverted Mean Gray Value (255 − MGV), which directly reflects β-gal activity. Data are expressed as mean ± SEM of two independent experiments in triplicate. Western blot analysis of the senescence markers p21, p53 and p16 was performed in untreated (CNTR) and OxxySlab -treated T24 ( B – D ), 5637 ( F – H ), and SV-HUC1 ( J – L ) cells. Protein levels were quantified by densitometry, normalized to GAPDH, and expressed as fold change relative to CNTR of three independent experiments (mean ± SEM). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01).
    Figure Legend Snippet: Senescence induction in BC cells following OxxySlab lysate treatment. Cell lines were treated with OxxySlab lysate (75–150 μg/mL) for 48 h. Senescent cells were identified by β-galactosidase staining (β-gal). Representative phase-contrast images of T24 ( A ), 5637 ( E ) and SV-HUC-1 ( I ) β-gal-positive cells (blue staining) are shown. Quantification of senescence was performed by measuring the Mean Gray Value of blue staining intensity using ImageJ software, with data expressed as Inverted Mean Gray Value (255 − MGV), which directly reflects β-gal activity. Data are expressed as mean ± SEM of two independent experiments in triplicate. Western blot analysis of the senescence markers p21, p53 and p16 was performed in untreated (CNTR) and OxxySlab -treated T24 ( B – D ), 5637 ( F – H ), and SV-HUC1 ( J – L ) cells. Protein levels were quantified by densitometry, normalized to GAPDH, and expressed as fold change relative to CNTR of three independent experiments (mean ± SEM). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01).

    Techniques Used: Staining, Software, Activity Assay, Western Blot

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    Article Title: Induction of CDK inhibitor p21 gene as a new therapeutic strategy against pulmonary fibrosis.
    Article Snippet: Inoshima, Ichiro, Kazuyoshi Kuwano, Naoki Hamada, Michihiro Yoshimi, Takashige Maeyama, Naoki Hagimoto, Yoichi Nakanishi, and Nobuyuki Hara.. Induction of CDK inhibitor p21 gene as a new therapeutic strategy against pulmonary fibrosis.. Am J Physiol Lung Cell Mol Physiol 286: L727–L733, 2004; 10.1152/ajplung.00209.

    Article Title: Integrin β1 subunit overexpressed in the SMMC‐7721 cells regulates the promoter activity of p21<sup>CIP1</sup> and enhances its transcription
    Article Snippet: .. The membranes were blocked in 5% non-fat dry milk in PBST (PBS+0.05% Tween 20) and incubated with mouse anti-human integrin L1 subunit McAb (BD Transduction Laboratories, 1:500^1:1000) or rabbit anti-human p21 polyclonal antibody (Santa Cruz, 1:500) diluted in 5% milk in PBST overnight at room temperature. .. Following three washes in PBST, the blots were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (Calbiochem) diluted in 1:500.

    Article Title: Increased expression of integrin beta1 subunit enhances p21WAF1/Cip1 transcription through the Sp1 sites and p300-mediated histone acetylation in human hepatocellular carcinoma cells.
    Article Snippet: Integrins, a class ofmembrane receptors, aremajor players in transmitting themechanical force across the plasma membrane.We previously reported that overexpression of integrin b1 subunit imposed a growth inhibitory effect on the hepatocellular carcinoma cell line SMMC-7721 through transcriptional activation of p21 gene.. In this study, we further determined the molecular mechanisms underlying p21 expression induced by integrin b1 overexpression.. We report herein that overexpression of integrin b1 subunit upregulates p21 transcription through a p53-independent pathway.

    Article Title: Exploring the Anticancer Potential of the Multistrain Probiotic Formulation OxxySlab in Bladder Cancer Cell Lines
    Article Snippet: Proteins were electroblotted onto 0.45 μm nitrocellulose membranes (BioRad). .. Following incubation with 5% non-fat dry milk in Tris-buffered saline for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies: goat anti-human vimentin polyclonal antibody (Chemicon International, Temecula, CA, USA; dilution 1:100), mouse anti-human E-cadherin monoclonal antibody (Cell Signaling Technology; dilution 1:1000), rabbit anti-human β-catenin monoclonal antibody (Cell Signaling Technology; dilution 1:1000), rabbit anti-human p21 polyclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA dilution 1:1000); rabbit anti-human phospho-Nrf2 monoclonal antibody (S40) (1:2000, Abcam, Cambridge, UK); rabbit anti-human p16 polyclonal antibody (Santa Cruz Biotechnology, dilution 1:200); mouse anti-human p53 monoclonal antibody (Santa Cruz Biotechnology, dilution 1:1000); mouse anti-human GAPDH monoclonal antibody (Immunological Sciences, Rome, Italy; dilution 1:1000). .. As secondary antibodies, peroxidase-conjugated anti-rabbit, and anti-mouse IgG antibodies (1:2000 dilution) were purchased from Sigma-Aldrich, and peroxidase-conjugated anti-goat IgG antibody (1:5000 dilution) was purchased from Santa Cruz Biotechnology.

    Western Blot:

    Article Title: Bursal Hexapeptide, A Potential Immunomodulator, Inhibits Tumor Cells Proliferation via p53 Signaling Pathway.
    Article Snippet: Background: The Bursa of Fabricius (BF) is acknowledged as the central humoral immune organ unique to birds.. Bursal Hexapeptide (BHP, AGCCNG) is a recently reported bursal-derived bioactive peptide.. However, there are few reports of the molecular basis of the mechanism on immune induction and potential antitumor activity of BHP.

    Saline:

    Article Title: Exploring the Anticancer Potential of the Multistrain Probiotic Formulation OxxySlab in Bladder Cancer Cell Lines
    Article Snippet: Proteins were electroblotted onto 0.45 μm nitrocellulose membranes (BioRad). .. Following incubation with 5% non-fat dry milk in Tris-buffered saline for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies: goat anti-human vimentin polyclonal antibody (Chemicon International, Temecula, CA, USA; dilution 1:100), mouse anti-human E-cadherin monoclonal antibody (Cell Signaling Technology; dilution 1:1000), rabbit anti-human β-catenin monoclonal antibody (Cell Signaling Technology; dilution 1:1000), rabbit anti-human p21 polyclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA dilution 1:1000); rabbit anti-human phospho-Nrf2 monoclonal antibody (S40) (1:2000, Abcam, Cambridge, UK); rabbit anti-human p16 polyclonal antibody (Santa Cruz Biotechnology, dilution 1:200); mouse anti-human p53 monoclonal antibody (Santa Cruz Biotechnology, dilution 1:1000); mouse anti-human GAPDH monoclonal antibody (Immunological Sciences, Rome, Italy; dilution 1:1000). .. As secondary antibodies, peroxidase-conjugated anti-rabbit, and anti-mouse IgG antibodies (1:2000 dilution) were purchased from Sigma-Aldrich, and peroxidase-conjugated anti-goat IgG antibody (1:5000 dilution) was purchased from Santa Cruz Biotechnology.



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    Senescence induction in BC cells following OxxySlab lysate treatment. Cell lines were treated with OxxySlab lysate (75–150 μg/mL) for 48 h. Senescent cells were identified by β-galactosidase staining (β-gal). Representative phase-contrast images of T24 ( A ), 5637 ( E ) and SV-HUC-1 ( I ) β-gal-positive cells (blue staining) are shown. Quantification of senescence was performed by measuring the Mean Gray Value of blue staining intensity using ImageJ software, with data expressed as Inverted Mean Gray Value (255 − MGV), which directly reflects β-gal activity. Data are expressed as mean ± SEM of two independent experiments in triplicate. Western blot analysis of the senescence markers <t>p21,</t> p53 and p16 was performed in untreated (CNTR) and OxxySlab -treated T24 ( B – D ), 5637 ( F – H ), and SV-HUC1 ( J – L ) cells. Protein levels were quantified by densitometry, normalized to GAPDH, and expressed as fold change relative to CNTR of three independent experiments (mean ± SEM). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01).
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    Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of <t>p21,</t> Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; <t>p21,</t> cyclin‑dependent kinase inhibitor <t>1A;</t> Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.
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    Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of <t>p21,</t> Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; <t>p21,</t> cyclin‑dependent kinase inhibitor <t>1A;</t> Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.
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    Santa Cruz Biotechnology rabbit polyclonal anti human p21 antibodies
    Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of <t>p21,</t> Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; <t>p21,</t> cyclin‑dependent kinase inhibitor <t>1A;</t> Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.
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    Image Search Results


    Senescence induction in BC cells following OxxySlab lysate treatment. Cell lines were treated with OxxySlab lysate (75–150 μg/mL) for 48 h. Senescent cells were identified by β-galactosidase staining (β-gal). Representative phase-contrast images of T24 ( A ), 5637 ( E ) and SV-HUC-1 ( I ) β-gal-positive cells (blue staining) are shown. Quantification of senescence was performed by measuring the Mean Gray Value of blue staining intensity using ImageJ software, with data expressed as Inverted Mean Gray Value (255 − MGV), which directly reflects β-gal activity. Data are expressed as mean ± SEM of two independent experiments in triplicate. Western blot analysis of the senescence markers p21, p53 and p16 was performed in untreated (CNTR) and OxxySlab -treated T24 ( B – D ), 5637 ( F – H ), and SV-HUC1 ( J – L ) cells. Protein levels were quantified by densitometry, normalized to GAPDH, and expressed as fold change relative to CNTR of three independent experiments (mean ± SEM). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01).

    Journal: Antioxidants

    Article Title: Exploring the Anticancer Potential of the Multistrain Probiotic Formulation OxxySlab in Bladder Cancer Cell Lines

    doi: 10.3390/antiox14111282

    Figure Lengend Snippet: Senescence induction in BC cells following OxxySlab lysate treatment. Cell lines were treated with OxxySlab lysate (75–150 μg/mL) for 48 h. Senescent cells were identified by β-galactosidase staining (β-gal). Representative phase-contrast images of T24 ( A ), 5637 ( E ) and SV-HUC-1 ( I ) β-gal-positive cells (blue staining) are shown. Quantification of senescence was performed by measuring the Mean Gray Value of blue staining intensity using ImageJ software, with data expressed as Inverted Mean Gray Value (255 − MGV), which directly reflects β-gal activity. Data are expressed as mean ± SEM of two independent experiments in triplicate. Western blot analysis of the senescence markers p21, p53 and p16 was performed in untreated (CNTR) and OxxySlab -treated T24 ( B – D ), 5637 ( F – H ), and SV-HUC1 ( J – L ) cells. Protein levels were quantified by densitometry, normalized to GAPDH, and expressed as fold change relative to CNTR of three independent experiments (mean ± SEM). Statistical significance was assessed by one-way ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01).

    Article Snippet: Following incubation with 5% non-fat dry milk in Tris-buffered saline for 1 h at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies: goat anti-human vimentin polyclonal antibody (Chemicon International, Temecula, CA, USA; dilution 1:100), mouse anti-human E-cadherin monoclonal antibody (Cell Signaling Technology; dilution 1:1000), rabbit anti-human β-catenin monoclonal antibody (Cell Signaling Technology; dilution 1:1000), rabbit anti-human p21 polyclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA dilution 1:1000); rabbit anti-human phospho-Nrf2 monoclonal antibody (S40) (1:2000, Abcam, Cambridge, UK); rabbit anti-human p16 polyclonal antibody (Santa Cruz Biotechnology, dilution 1:200); mouse anti-human p53 monoclonal antibody (Santa Cruz Biotechnology, dilution 1:1000); mouse anti-human GAPDH monoclonal antibody (Immunological Sciences, Rome, Italy; dilution 1:1000).

    Techniques: Staining, Software, Activity Assay, Western Blot

    Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of p21, Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; p21, cyclin‑dependent kinase inhibitor 1A; Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.

    Journal: Molecular medicine reports

    Article Title: Growth inhibition effects of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on colorectal carcinoma cells and colon carcinoma-bearing mice.

    doi: 10.3892/mmr.2016.4950

    Figure Lengend Snippet: Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of p21, Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; p21, cyclin‑dependent kinase inhibitor 1A; Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.

    Article Snippet: Immunoblots of 50 μg total protein were probed with primary antibodies overnight as follows: Rabbit anti-human polyclonal cyclin-dependent kinase inhibitor 1A (p21, Cip1) (p21; cat. no. BA0272; dilution, 1:200; Boster Bio-Engineering Ltd., Wuhan, China), rabbit anti-human polyclonal B-cell lymphoma 2 (Bcl-2; cat. no. ZS-492; dilution, 1:300; Zhongshan Golden Bridge Biotechnology, Co., Ltd., Beijing, China), mouse anti-mouse monoclonal Bcl-2-associated X protein (Bax; cat. no. ZS-7480; dilution, 1:300; Zhongshan Golden Bridge Biotechnology, Co., Ltd.), rabbit anti-human polyclonal nuclear factor κ-light-chain enhancer of activated B cells (NF-κB) p65 phosphorylated (p)-Ser536 (cat. no. 11014; dilution, 1:500; Signalway Antibody LLC., College Park, MD, USA), rabbit anti-human polyclonal nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor α (IκBα), rabbit anti-human polyclonal survivin (cat. no. sc-10811; dilution, 1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and β-actin (cat. no. sc-130656; dilution, 1:500; Santa Cruz Biotechnology, Inc.).

    Techniques: Western Blot, Control

    Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of p21, Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; p21, cyclin‑dependent kinase inhibitor 1A; Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.

    Journal: Molecular medicine reports

    Article Title: Growth inhibition effects of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on colorectal carcinoma cells and colon carcinoma-bearing mice.

    doi: 10.3892/mmr.2016.4950

    Figure Lengend Snippet: Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of p21, Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; p21, cyclin‑dependent kinase inhibitor 1A; Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.

    Article Snippet: Immunoblots of 50 μg total protein were probed with primary antibodies overnight as follows: Rabbit anti-human polyclonal cyclin-dependent kinase inhibitor 1A (p21, Cip1) (p21; cat. no. BA0272; dilution, 1:200; Boster Bio-Engineering Ltd., Wuhan, China), rabbit anti-human polyclonal B-cell lymphoma 2 (Bcl-2; cat. no. ZS-492; dilution, 1:300; Zhongshan Golden Bridge Biotechnology, Co., Ltd., Beijing, China), mouse anti-mouse monoclonal Bcl-2-associated X protein (Bax; cat. no. ZS-7480; dilution, 1:300; Zhongshan Golden Bridge Biotechnology, Co., Ltd.), rabbit anti-human polyclonal nuclear factor κ-light-chain enhancer of activated B cells (NF-κB) p65 phosphorylated (p)-Ser536 (cat. no. 11014; dilution, 1:500; Signalway Antibody LLC., College Park, MD, USA), rabbit anti-human polyclonal nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor α (IκBα), rabbit anti-human polyclonal survivin (cat. no. sc-10811; dilution, 1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and β-actin (cat. no. sc-130656; dilution, 1:500; Santa Cruz Biotechnology, Inc.).

    Techniques: Western Blot, Control